Cytotoxic Properties of Some Medicinal Plant Extracts from …
Ethanolic extract of Ferula assa-foetida L. has significant cytotoxicity effect on Hela cell line in concentration range between 10 mg/ml to 2.5 mg/ml by using MTT assay compared with control. This extract also exerts the high cytotoxicity against HeLa cell in 2.5 mg/ml concentration with 89.65 and 80.49 percent of cell growth inhibition.
Concentration Matters: A Comprehensive Guide to Plant Extract
The determination of extract concentration is a critical step in the process of preparing plant extracts for various applications. Accurate measurement of the concentration ensures that the extracts are standardized and can be reliably used in research, pharmaceuticals, or other industries.
Avoiding pitfalls in determining antimicrobial activity of plant
Because many aqueous plant extracts contain anti-oxidant compounds, the efficacy of plant extracts used traditionally may not be based on inhibiting microbial growth …
Phytochemical Analysis, Antioxidant and Antimicrobial …
Four different concentrations of plant extract (0.5-4 mg/mL) were tested with 3 replications. An aliquot of 40 µL of plant extract was inoculum in each well, and ciprofloxacin (5μg/disk) was used as positive control. After 12-15 min of diffusion time at room temperature, the inoculated plates were incubated at 37°C for 48 h.
How can I determine plant extract concentration?
Do you want to make water and ethanol extracts of plant material and test the extracts for inhibition of something by adding the extracted material in DMSO at a final concentration of 0.5%...
Plant Extract
87 rowsPlant extracts are mixtures of aromatic secondary metabolites such as phenols, phenolic acids, terpene, terpenoid, flavonoids, and sometimes fatty acids that are usually …
Innovations in Concentration: Emerging Trends in Plant Extract …
Discover the essentials of plant extract concentration with this comprehensive guide. Learn why concentration matters, explore various concentration methods, and understand the factors influencing the process. Gain insights on the necessary equipment, safety measures, and quality control for reliable results. Delve into the applications and real-world …
The Art of Extraction: A Step-by-Step Guide to Methanol Plant …
- The dried extract is stored in airtight containers, often under low temperature and protected from light to preserve the integrity of the compounds. 10. Quality Control: - The final extract is analyzed for its chemical composition, purity, and concentration to ensure it meets the desired specifications for further use.
Antibacterial and antibiofilm activity of acetone …
Plant extracts may have good activity per se or may be sources of effective antimicrobial compounds which can act against planktonic and/or biofilms of pathogens. We determined the antimicrobial efficacy and …
Innovations in Concentration: Emerging Trends in Plant Extract …
Discover the essentials of plant extract concentration with this comprehensive guide. Learn why concentration matters, explore various concentration methods, and understand the factors influencing the process. Gain insights on the necessary equipment, safety measures, and quality control for reliable results. Delve into the applications and real-world case studies showcasing …
Pesticidal Plant Extracts Improve Yield and Reduce Insect …
Data on the effect of plant extract concentration has been reported with expected dose response trends on crop yield when applying plant treatments at 0.1, 1.0, and 10% w/v (Mkindi et al., 2017). All data presented here are based on the application of 10% w/v as this was determined to be the most effective concentration for reducing insect ...
Evaluation of different high doses aqueous plant extracts for …
Aedes aegypti mosquito eggs, collected from NIFA's Medical Entomology laboratory in Peshawar, were exposed to various plant extract concentrations in plastic cups, with about 100 eggs per cup. The control group was subjected to tap water. Each concentration was replicated thrice. Data on egg hatching was recorded, with hatched eggs separated ...
Plant Extracts Rich in Polyphenols as Potent Modulators in …
Plant extracts were added to the culture media to obtain a final concentration of dry matter in the range from 0.25 to 10 mg extract dry matter/mL, which are concentrations of extracts expected to be found under physiological conditions (33, 36). One control sample remained without any plant extract.
Plant Extract
Plants and plant extracts have been explored under different environmental conditions to successfully produce metallic nanostructures (Table 3).Plant extract is the most commonly used agent to produce metallic NPs. Nevertheless, experiments have demonstrated that in some cases live plants show the ability to absorb metals from the outside and accumulate them in their …
Concentration Matters: A Comprehensive Guide to Plant …
The determination of extract concentration is a critical step in the process of preparing plant extracts for various applications. Accurate measurement of the concentration …
Concentrating on Excellence: A Comprehensive Guide to Plant Extract …
1. Importance of Plant Extract Concentration Plant extracts have been a cornerstone of traditional medicine and are increasingly valued in modern pharmaceuticals, cosmetics, and food industries due to their rich bioactive compounds. The concentration of plant extracts is a critical process that enhances their efficacy, stability, and ...
Plant Extract
23 rowsPlant extracts are considered to be the most pressing sources of biomolecules, which can be screened from plant parts. The extraction of such biomolecules through medicinal …
Antibacterial Efficacy and Minimum Inhibitory Concentrations …
The Minimum inhibitory concentration was determined by two fold serial dilution methods. The Concentration of plant sample of about 551.099μg of Azadirachta indica, 681.175μg of Centella asiatica, ... Then, 5µl of plant extract (ethanolic plant extract) was added in the well by using micro pipette. Methanol added well considered as a control ...
Antibacterial Activity of Four Plant Extracts Extracted from
To conduct the time–kill assay using the in vitro model, each plant extract was added to 1mL cecum solution to obtain 1/2, 1, 2, 3, and 4 × MIC of each plant extract. Concentrations were based on the broth microdilution results which identified the minimum concentrations of plant extracts that inhibit bacteria in broth after 24 h.
Phytochemicals: Extraction, Isolation, and …
In summary, plant extracts showed strong antioxidant capacity both in vitro and in vivo, and the extracts can be considered a good source of natural antioxidants and antimicrobials.
Antibacterial and antibiofilm activity of acetone leaf …
Acetone was used as the solvent of choice to extract the powdered leaves as well as to prepare the plant concentrations for the bioassays. Extraction with acetone is considered the best choice because it can extract compounds of a wide range of polarities, it is nontoxic to bioassay systems and easy to remove from extracts [ 39 ].
Effect of Plant Extracts Formulated in Different Ointment …
Agar ditch method was used for the initial screening of the antibacterial activity of the plant extracts. 0.5 g of extract in 10 ml nutrient agar was used (5% extract). Minimum inhibitory concentration (MIC) of the individual plant extracts was determined by plate dilution technique.
Potent Cytotoxicity of Four Cameroonian Plant Extracts on …
SRB assay on HeLa cells to evaluate the effect of the four plant extracts: (A) Xylopia aethiopica (XA), (B) Imperata cylindrica (IC), (C) Echinops giganteus (EG) and (D) Dorstenia psilurus (DP) on the cell growth after 48 h (X-axis: log concentrations of extracts from 1 to 200 (µg/mL) and Y-axis: the percentage of normalized absorbance).While (E): The …
Antibacterial and Antibiofilm Activity of Methanolic Plant …
Briefly, 100 μL of Mueller-Hinton Broth (Difco) plus different concentrations of plant extracts was prepared and transferred to each microplate well to obtain dilutions of the active extract, ranging from 1.0 to 25 mg/mL.
Antimicrobial Properties and Mechanism of Action …
The Minimum Inhibitory Concentration (MIC) of plant extracts against the test microorganisms. It is fundamental to develop a better understanding of the antimicrobial mechanism of plant crude extracts on the …
Plant extracts versus a fungus, why lower …
We conducted a laboratory experiment (two trials) on the effect of plant extract against rice blast fungus. The study involved 0, 25, 50, 75, and concentrations of ethanolic extracts against ...
Antiangiogenic Activity of Philippine Medicinal Plant Extracts Assessed
the plant extract derived fro m Callistemon . ... concentration of the extract since, unlike . those subjects where malformations and . mortalities have already been observed after .
Antimicrobial Properties and Mechanism of Action of Some Plant …
This study supports previous findings in the literature that the antimicrobial activities have a direct relation to increasing the extracts concentration (%) (Bhalodia and Shukla, 2011).Significant antimicrobial effects, expressed as MIC of each plant extract against test microorganism is given in Table 3.The data revealed variability in the MIC among plant …
Preliminary Screening of Antibacterial Activity Using Crude …
Crude petroleum ether extract, ethyl acetate extract and methanol extract from the leaves, stems and flowers of the plant were prepared using a cold extraction technique. These extracts were tested at concentrations ranging from 4 mg/disc to 0.017 mg/disc against methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus ...
Green synthesised copper and copper oxide based nanomaterials using
Many parameters, including pH, temperature, the concentration of metal ions, and the amount of phytochemicals in the plant extract, influence the reduction potential. As a result, a single plant extract can yield a variety of nanoparticles. Ansilin et al. used neem leaf aqueous extract to make CuO nanoparticles.